This protocol was adapted from the protocol of Rachel Bober (RachelBober) written on 10.12.2024.

Design

Here, I tested 3’-end libraries sequencing results.

This protocol was adapted from the protocol of Rachel Bober (RachelBober) written on 26.11.24. The aim is to compare symbiont species of P. astreoides across treatments in the translocation experiment. For this:

1. DNA was extracted from acetone-fixed zooxantellae from each coral sample
2. DNA was amplified with ITS2 primers
3. PCR products were visualised using gel electrophoresis
4. The bands were cut from the gel and cleaned

Using the Zymo Quick-RNA Miniprep Kit on Stylophora pistillata samples across depths.